Isolation, characterization and antimicrobial activity of a Streptomyces strain isolated from deteriorated wood (2025)

Streptomyces hundungensis sp. nov., a novel actinomycete with antifungal activity and plant growth promoting traits

The Journal of Antibiotics, 2013

A novel actinobacterium MBRL 251 T , isolated from a sample collected from a limestone quarry at Hundung, Manipur, India, was characterized using a polyphasic approach. The 16S ribosomal RNA gene sequence of strain MBRL 251 T showed closest similarities with Streptomyces xanthochromogenes NRRL B-5410 T (99.6%) and Streptomyces michiganensis NBRC 12797 T (99.6%). The DNA relatedness between MBRL 251 T and S. xanthochromogenes NBRC 12828 T , and S. michiganensis NBRC 12797 T was 46.6% and 40.7%, respectively. Strain MBRL 251 T contained LL-diaminopimelic acid as the diagnostic diamino acid, with glucose and xylose as the main cell wall sugars, whereas small amounts of galactose, mannose, rhamnose and ribose were also detected in the whole-cell wall hydrolysates. The major fatty acids identified were anteiso-C 15:0 (35.1%), iso-C 16:0 (21.1%) and anteiso-C 17:1 (13.2%). The predominant menaquinones detected were MK-9(H 6) and MK-9(H 8), whereas the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannosides. The G þ C content of the genomic DNA was 72.3%. The phenotypic and genotypic data showed that strain MBRL 251 T merits the recognition as a representative of a novel species of the genus Streptomyces. It is proposed that the isolate should be classified in the genus Streptomyces as a novel species, Streptomyces hundungensis sp. nov. The type strain is MBRL 251 T (¼ JCM 17577 T ¼ KCTC 29124 T).

New and bioactive compounds from Streptomyces strains residing in the wood of Celastraceae

Planta, 2002

Wood from three different plants of the Celastraceae growing in their natural habitats in Brazil (Maytenus aquifolia Mart.) and South Africa [Putterlickia retrospinosa van Wyk and Mostert, P. verrucosa (E. Meyer ex Sonder) Szyszyl.] was established as a source of endophytic bacteria using a medium selective for actinomycetes. Two isolates were identified as Streptomyces setonii and S. sampsonii whereas two others were not assignable to any of the known Streptomyces species. They were preliminarily named Streptomyces Q21 and Streptomyces MaB-QuH-8. The latter strain produces a new chloropyrrol and chlorinated anthracyclinone. The chloropyrrol showed high activity against a series of multiresistent bacteria and mycobacteria.

Screening the Egyptian desert actinomycetes as candidates for new antimicrobial compounds and identification of a new desert Streptomyces strain

In a screening program to study the antimicrobial activities of desert actinomycetes as potential producers of active metabolites, 75 actinomycete strains were isolated from the Egyptian desert habitats and tested. Out of the isolated 75 organisms, 32 (42.67%) showed activity against the used test organisms. The antimicrobial activities of the active desert actinomycete strains were classified into four groups according to their spectrum of activity on different groups of test organisms and it was found that 43.75% of the active isolates have activity against Gram-positive bacteria only, 28.13% have activity against both Gram-positive and Gram-negative bacteria, 15.63% have activity against Gram-positive bacteria and yeast, 12.50% have activity against Gram-positive, Gram-negative bacteria and yeast. The most potent actinomycete strain, designated D332, was selected for further studies including its identification and isolation of its active compound. Strain D332 was identified by studying its morphology, chemotaxonomy, biochemical characteristics and phylogenetic analysis of the 16S rRNA gene sequence. All phenotypic and genotypic characteristics were consistent with the classification of strain D332 to genus Streptomyces where it formed a distinct phyletic line in the Streptomyces 16S rRNA gene tree. On the other hand, the culture broth of strain D332 was extracted with ethyl acetate after fermentation for the production of the active compound then, the crude extract was partially purified by thin layer chromatography using a solvent system composed of heptane: ethyl acetate (3: 2). The results revealed that strain D332 produced one major compound active against Gram-positive and Gramnegative bacteria and yeasts.

Antimicrobial and Antiviral Activities of An Actinomycete (Streptomyces Sp.) Isolated From a Brazilian Tropical Forest Soil

World Journal of …, 2004

A promising producer of bioactive compounds isolated from a Brazilian tropical soil was tested for its range of antimicrobial activities. Strain 606, classified as Streptomyces sp., could not be identified up to species level, suggesting a possible new taxon. The supernatant and 10 extracts and fractions, obtained by extraction and chromatographic techniques, presented antimicrobial activity using antibiograms. The methanolic fraction was highly active against pathogenic bacteria, phytopathogenic fungi and the human pathogenic yeast Candida albicans. It also possessed high antiviral activity inhibiting the propagation of an acyclovir-resistant herpes simplex virus type 1 strain on HEp-2 cells at non-cytotoxic concentration. The strong cytotoxic effect suggests an antitumour action.

Isolation, Identification and Characterization of Antimicrobial Activity Exhibiting Actinomycete Streptomyces paradoxus Strain KUASN-7 from Soil

International Journal of Current Microbiology and Applied Sciences, 2021

Isolation and Identification of Actinomycetes from Mangrove Soil and Extraction of Secondary Metabolites for Antibacterial Activity

The mangrove ecosystem of India is an extensively unexplored source for actinomycetes with the potential to produce secondary metabolites of biological importance. In this study, twenty two actinomycetes were isolated from different soil samples collected from the Bhitarkanika mangrove forest along Odisha coast, India. These isolates were identified as Streptomyces sp. based on their morphological, physiological and biochemical characteristics as described in the International Streptomyces Project. Out of twenty two actinomycetes (designated as BSA-1 to BSA-22) isolates, only four isolates (BSA-5, BSA-10, BSA-11 and BSA-15) displayed significant antimicrobial Original Research Article Priyadarshini et al.; BBJ, 12(2): 1-13, 2016; Article no.BBJ.24102 2 properties in term of antagonistic activity against six human pathogenic bacterial strains (Staphylococcus aureus, Shigella flexneri, Bacillus licheniformis, Bacillus brevis, Pseudomonas aeruginosa and Escherichia coli). All these isolates exhibited excellent antimicrobial activity in a range of 14.0-22.0 mm as inhibition zone against the above studied human pathogens with highest activity displayed by the isolate BSA-11. The isolate, Streptomyces sp. BSA-11 was further identified up to the species level by 16S rRNA gene sequence analysis. The BLAST analysis confirmed that Streptomyces sp. BSA-11 was homologous to Streptomyces himastatinicus of order Actinomycetles and class Actinobacteria. The novel actinomycete, Streptomyces himastatinicus BSA-11 from Bhitarkanika has the ability to produce extracellular potent bioactive compounds which can be a potential source of many antimicrobials.

ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES GOUSE BASHA SHEIK 1* , MUAZZAM SHERIFF MAQBUL

International Journal of Pharmacy and Pharmaceutical Sciences , 2017

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteria Methods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods. Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm). Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES Original Article GOUSE BASHA SHEIK 1* , MUAZZAM SHERIFF MAQBUL 2 , GOKUL SHANKAR S 3 , RANJITH MS 4

International Journal of Pharmacy and Pharmaceutical Sciences, 2017

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteria Methods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods. Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm). Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

INHIBITION OF SOME HUMAN BACTERIAL PATHOGENS USING Streptomyces sp. SD5 OBTAINED FROM SOIL SAMPLE FROM JEDDAH

Journal of Experimental Biology and Agricultural Sciences

For decades, antibiotics are wonder drugs which treat different microbes and human pathogens such as pneumonia, tuberculosis and gonorrhea which are harder and difficult to treat. Due to miss use of antibiotics in agriculture and animal husbandry, antibiotics are becoming less effective and microbes became more resistant. This resistant increased every second, thus this study aimed to produce active antibiotic from soil actinomycetes which might play a highly significant role in medicine. About 15 bacterial isolates were obtained on starch nitrate agar medium from different soil samples. They were screened for antibacterial production against 5 different human pathogens, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Methicillin-resistant Staphylococcus aureus (MRSA). The most active isolate was SD5 which showed the highest inhibition against E. coli, E. faecalis and MRSA was morphologically examined and characterized. Using molecular identification technique, it was reported that isolate SD5 belonging to genus Streptomyces and was similar to Streptomyces geysiriensis with 97% similarity and to Streptomyces sp. JSM147777 with 95% similarity. Maximum production of the antimicrobial agent was determined by measuring the diameter of inhibition zone using starch nitrate broth, prepared at pH 6.5 and incubation temperature at 30°C for 5 days. The antimicrobial agent was extracted by using three different organic solvents (ethyl acetate, n-Butanol and Petroleum ether). The best solvent was ethyl acetate which gave maximum inhibition against E. faecalis, E. coli and Methicillin-resistant S. aureus. In conclusion, actinomycetes especially

Broad-spectrum antimicrobial activity of wetland-derived Streptomyces sp. ActiF450

EXCLI Journal, 2020

The increased incidence of invasive infections and the emerging problem of drug resistance particularly for commonly used molecules have prompted investigations for new, safe and more effective microbial agents. Actinomycetes from unexplored habitats appear as a promising source for novel bioactive compounds with a broad range of biological activities. Thus, the present study aimed to isolate effective wetland-derived actinomycetes against major pathogenic fungi and bacteria. Water samples were collected from various locations of Fetzara Lake, Algeria. Thereafter, an actinomycete designated ActiF450 was isolated using starch-casein-agar medium. The antimicrobial potential of the newly isolated actinomycete was screened using the conventional agar cylinders method on Potato Dextrose Agar (PDA) against various fungal and bacterial pathogens. A wetland-derived Streptomyces sp. Actif450 was identified as Streptomyces malaysiensis based on its physiological properties, morphological char...